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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
Comments |
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|---|---|---|---|---|---|---|---|---|---|---|
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YCe1901 HC_Kan_PGKpA_p22 Resource Report Resource Website |
RRID:Addgene_100683 | PGKpA | Homo sapiens | Kanamycin | PMID:28418644 | mouse phosphoglucokinase polyA. Terminator. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
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YCe1911 HC_Kan_Insulator_FB_p2 Resource Report Resource Website |
RRID:Addgene_100686 | Insulator_FB | Synthetic | Kanamycin | PMID:28418644 | FII/BEAD-A (FB) element. (STEMCELLS2008;26:3257�3266 ). FII/BEAD-A (FB) contains the minimal enhancer-blocking components of the chicken b-globin 5'HS4 insulator and a homologous region from the human T-cell receptor BEAD-1 insulator IMPORT NOTES: Special character found in field(Comments) | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
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YCe2038 HC_Kan_CMVp_p18 Resource Report Resource Website |
RRID:Addgene_100690 | CMV promoter | Other | Kanamycin | PMID:28418644 | Strong constitutive promoter : Human�cytomegalovirus�(CMV) immediate early enhancer and�promoter IMPORT NOTES: Special character found in field(Comments) | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
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YCe2040 HC_Kan_PuroR_p21 Resource Report Resource Website |
RRID:Addgene_100691 | PuroR | Other | Kanamycin | PMID:28418644 | Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
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YCe2046 HC_Kan_PuroR_p15 Resource Report Resource Website |
RRID:Addgene_100692 | PuroR | Other | Kanamycin | PMID:28418644 | Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
|
YCe2052 HC_Kan_DmrC_p9 Resource Report Resource Website |
RRID:Addgene_100693 | DmrC | Synthetic | Kanamycin | PMID:28418644 | DmrC is a chemically inducible dimerization domain.It dimerizes with DmrA in presence of heterodimerizer. iDimerizeTM Inducible Heterodimer System - Clontech | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
|
YCe2746 HC_Kan_Linker1_p8 Resource Report Resource Website |
RRID:Addgene_100699 | Linker1 | Synthetic | Kanamycin | PMID:28418644 | Flexible peptide linker: SGLRSGSGGGSASGGSG . PROTEIN SCIENCE 2013 VOL 22:153�167 IMPORT NOTES: Special character found in field(Comments) | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
|
YCe2058 HC_Kan_Kozak_ATG_Palm_p6 Resource Report Resource Website |
RRID:Addgene_100694 | Kozak_ATG_Palm | Homo sapiens | Kanamycin | PMID:28418644 | N-terminal GAP43-palmitoylation sequence. Petide tag for targeting the protein to the plasma membrane | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
|
YCe2060 HC_Kan_Linker3_p20 Resource Report Resource Website |
RRID:Addgene_100695 | Linker3 | Synthetic | Kanamycin | PMID:28418644 | Flexible peptide linker: GGGSGGGGSAS | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
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YCe2064 HC_Kan_p2A_p20 Resource Report Resource Website |
RRID:Addgene_100696 | P2A | Other | Kanamycin | PMID:28418644 | p2A-Porcine teschovirus-1: self-cleaving� 2A peptide. Nat.Biotechnol.2004. 22, 589�594 IMPORT NOTES: Special character found in field(Comments) | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:55 | 0 | |
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B52 (empty plasmid backbone to express 2 sgRNAs) Resource Report Resource Website |
RRID:Addgene_100708 | Ampicillin | PMID:28890334 | Backbone Size:2633; Vector Backbone:B52 (MLM3636 plasmid backbone modified to express 2 sgRNAs); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:55 | 0 | ||||
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B52 + SPRTN sgSTOP Resource Report Resource Website |
RRID:Addgene_100709 | sgSTOP targeting SPRTN (cloned using BbsI) | Homo sapiens | Ampicillin | PMID:28890334 | Vector Backbone:B52 plasmid expressing SPRTN sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:55 | 0 | ||
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YCe2725 HC_Kan_conn_BsaI-2_p24 Resource Report Resource Website |
RRID:Addgene_100669 | EMMA toolkit Assembly connector for second step assembly | Synthetic | Kanamycin | PMID:28418644 | DNA element with no function and internal BsaI site generating a ATCC overhang upon cleavage. It is used in the first step BsmBI-based Golden Gate along with HC_Kan_conn_BsaI-1_p1 to flank the vector with BsaI stes. Vectors built in the first BsmBI-based Golden Gate reaction will be flanked by BsaI sites that are used in a second step assembly using as entry vector HC_RFP-4 (the part-entry vector for position 4). See manuscript for futher information | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 | |
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YCe3762 HC_Kan_conn_Q-W Resource Report Resource Website |
RRID:Addgene_100660 | EMMA toolkit Assembly connector Q-W | Synthetic | Kanamycin | PMID:28418644 | DNA element with no function. It is used as a bridge to connect parts placed in position 16 and 23 of EMMA platform. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 | |
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YCe2733 HC_Kan_conn_W-Y Resource Report Resource Website |
RRID:Addgene_100665 | EMMA toolkit Assembly connector W-Y | Synthetic | Kanamycin | PMID:28418644 | DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 25 of EMMA platform. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 | |
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YCe3159 HC_Kan_conn_W-X Resource Report Resource Website |
RRID:Addgene_100666 | EMMA toolkit Assembly connector W-X | Synthetic | Kanamycin | PMID:28418644 | DNA element with no function. It is used as a bridge to connect parts placed in position 22 and 24 of EMMA platform. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 | |
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YCe2786 HC_Kan_conn_T-V Resource Report Resource Website |
RRID:Addgene_100662 | EMMA toolkit Assembly connector T-V | Synthetic | Kanamycin | PMID:28418644 | DNA element with no function. It is used as a bridge to connect parts placed in position 19 and 22 of EMMA platform. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 | |
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YCe3757 HC_Kan_conn_U-V Resource Report Resource Website |
RRID:Addgene_100663 | EMMA toolkit Assembly connector U-V | Synthetic | Kanamycin | PMID:28418644 | DNA element with no function. It is used as a bridge to connect parts placed in position 20 and 22 of EMMA platform. | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 | |
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B52 + TIMELESS sgSTOP Resource Report Resource Website |
RRID:Addgene_100713 | sgSTOP targeting TIMELESS (cloned using BbsI) | Homo sapiens | Ampicillin | PMID:28890334 | Vector Backbone:B52 plasmid expressing TIMELESS sgSTOP and containing an empty sgRNA-expression cassette; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin | 2022-04-22 03:14:55 | 0 | ||
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YCe1885 HC_Kan_Kozak-ATG-MLS_p6 Resource Report Resource Website |
RRID:Addgene_100676 | Kozak-ATG-MLS | Homo sapiens | Kanamycin | PMID:28418644 | Kozak-ATG-MLS (Mitochondrial Localization Sequence) | Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin | 2022-04-22 03:14:54 | 0 |
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