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Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-L
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 12 of EMMA platform.
Proper citation: RRID:Addgene_100645 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector A-C
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to fill in the gap between the backbone and parts placed in position 3 of EMMA platform.
Proper citation: RRID:Addgene_100640 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector N-R
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 13 and 18 of EMMA platform.
Proper citation: RRID:Addgene_100658 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Q-R
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 16 and 18 of EMMA platform.
Proper citation: RRID:Addgene_100659 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-W
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 23 of EMMA platform.
Proper citation: RRID:Addgene_100655 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-Y
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 25 of EMMA platform.
Proper citation: RRID:Addgene_100656 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector L-M
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 11 and 13 of EMMA platform.
Proper citation: RRID:Addgene_100652 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 19 of EMMA platform.
Proper citation: RRID:Addgene_100629 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 15 of EMMA platform.
Proper citation: RRID:Addgene_100625 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 16 of EMMA platform.
Proper citation: RRID:Addgene_100626 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Amp; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Receiver vector for EMMA. It is the plasmid used as receiver vector in the BsmBI golden gate reaction of EMMA and therefore provides the backbone of the resulting expression vector. It contains a ccdB gene which is replaced by the assembling parts.
Proper citation: RRID:Addgene_100637 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector A-B
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to fill in the gap between the backbone and parts placed in position 2 of EMMA platform.
Proper citation: RRID:Addgene_100638 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-C
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 3 of EMMA platform.
Proper citation: RRID:Addgene_100639 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 23 of EMMA platform.
Proper citation: RRID:Addgene_100633 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 20 of EMMA platform.
Proper citation: RRID:Addgene_100630 Copy
Species: Homo sapiens
Genetic Insert: RARRES3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100724 Copy
Species: Homo sapiens
Genetic Insert: C-KDEL
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: KDEL sequence at C terminal of the coding sequence cloned in position 7.
Proper citation: RRID:Addgene_100681 Copy
Species: Synthetic
Genetic Insert: mRuby2
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments: Fluorescent reporter - Red - ?ex (nm) = 559 ?em (nm) = 600 . doi: 10.1038/nmeth.2171
Proper citation: RRID:Addgene_100687 Copy
Species: Homo sapiens
Genetic Insert: Pim1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_100722 Copy
Species: Homo sapiens
Genetic Insert: MTMR1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET28; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: The MTMR1 construct is for structural determination and contains the PH-GRAM and PTPase domains (amino acids 95-607). PTPase activity was confirmed.
Proper citation: RRID:Addgene_100723 Copy
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