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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pGAL-flp(H305L)-NAT Resource Report Resource Website |
RRID:Addgene_160380 | FLP1 | Saccharomyces cerevisiae | Ampicillin | PMID: | NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids. | Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | H305L | 2022-12-10 12:02:51 | 0 |
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pGAL-SKI1-NAT Resource Report Resource Website |
RRID:Addgene_160382 | SKI1 | Saccharomyces cerevisiae | Ampicillin | PMID: | Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-12-10 12:02:51 | 0 | ||
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UPB-pancreas (UPB7) Resource Report Resource Website |
RRID:Addgene_180787 | hM4D(Gi) (partial ) | Synthetic | Ampicillin | PMID:35296859 | Backbone Marker:Edward Boyden; Backbone Size:6150; Vector Backbone:AAVrg-CAG-tdTomato-WPRE-SV40 (Addgene, Plasmid #59462); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-12-10 12:03:51 | 0 | ||
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PRS_8-ChrimsonR-tdTomato Resource Report Resource Website |
RRID:Addgene_192587 | ChrimsonR | Synthetic | Ampicillin | PMID: | Backbone Size:4095; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-12-10 12:04:17 | 0 | ||
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PRS_8-eOPN3-mScarlet Resource Report Resource Website |
RRID:Addgene_192596 | eOPN3 | Synthetic | Ampicillin | PMID: | Backbone Size:4098; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2022-12-10 12:04:17 | 0 | ||
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pCDNA3-EMTB-TurboID-V5 Resource Report Resource Website |
RRID:Addgene_190736 | ensconsin | Homo sapiens | Ampicillin | PMID:35952650 | Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Microtubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882) | 2022-12-10 12:04:13 | 0 | |
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hSyn-Aion-ts-mScarlet-ER Resource Report Resource Website |
RRID:Addgene_192577 | Aion | Synthetic | Ampicillin | PMID: | Backbone Size:4372; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156C, T159G, G163A, V242R, T246N, N258Q, E273S | 2022-12-10 12:04:17 | 0 | |
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pcDNA3-mCherry-Rheb(C181S) Resource Report Resource Website |
RRID:Addgene_192437 | mCherry-Rheb(C181S) | Homo sapiens | Ampicillin | PMID:35294906 | Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | Rheb Cys 181 mutated to Ser; abolishes C-terminal farnesylation. | 2022-12-09 12:04:16 | 0 | |
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Flag-Gsdmd Resource Report Resource Website 1+ mentions |
RRID:Addgene_80950 | Gsdmd | Mus musculus | Ampicillin | PMID:27383986 | Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-09 12:07:07 | 1 | ||
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RCAS(A) mLef1 (CT#1141) Resource Report Resource Website |
RRID:Addgene_14021 | Lef1 | Mus musculus | Ampicillin | PMID: | From Gabrielle Kardon. | Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin | 2022-12-09 12:02:14 | 0 | |
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pcDNA3-mCherry-TSC2(AA,3Q)-NLS Resource Report Resource Website |
RRID:Addgene_192445 | mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS | Homo sapiens | Ampicillin | PMID:35294906 | Backbone Size:5472; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in isoform 1) mutated to Ala; Lys 1614, Lys 1615, and Arg 1616 mutated to Gln. | 2022-12-09 12:04:17 | 0 | |
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pSCM167-Venus-VF-hADAM10(aa220-459) Resource Report Resource Website |
RRID:Addgene_189555 | truncated ADAM metallopeptidase domain 10 | Homo sapiens | Ampicillin | PMID: | Please note: Plasmid contains a Y316H mutation in hADAM10 compared to the NCBI reference sequence NP_001101.1. This mutation was present in the original DNA that was was PCR amplified from human placenta cDNA and is not known to affect plasmid function. | Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains aa 220-459 | 2022-12-09 12:04:11 | 0 |
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pCAG-mGSDMD1-251 Resource Report Resource Website |
RRID:Addgene_111565 | Gasdermin D | Mus musculus | Ampicillin | PMID:29539421 | Backbone is from Dr. Eiji Morita | Vector Backbone:pCAG-FOS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-09 12:00:42 | 0 | |
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pSCM167-Venus-VF-hSFRP1(aa181-314) Resource Report Resource Website |
RRID:Addgene_189550 | truncated secreted frizzled related protein 1 | Homo sapiens | Ampicillin | PMID: | Backbone Marker:Customized backbone from Fu lab ; Vector Backbone:pSCM167-Venus (Fu lab); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | contains aa181-314 | 2022-12-09 12:04:11 | 0 | |
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HA-mRIPK3 K51A-Flag/cHA-pTRIPZ vector Resource Report Resource Website |
RRID:Addgene_73704 | RIPK3 | Mus musculus | Ampicillin | PMID: | Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | K51A | 2022-12-09 12:06:45 | 0 | |
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HA-mRIPK3 D143N-Flag/cHA-pTRIPZ vector Resource Report Resource Website |
RRID:Addgene_73703 | RIPK3 | Mus musculus | Ampicillin | PMID: | Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | D143N | 2022-12-09 12:06:44 | 0 | |
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HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector Resource Report Resource Website |
RRID:Addgene_73702 | RIPK3 | Mus musculus | Ampicillin | PMID: | Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 448-AAAA-451 | 2022-12-09 12:06:44 | 0 | |
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HA-mRIPK3 D161N-Flag/cHA-pTRIPZ vector Resource Report Resource Website |
RRID:Addgene_73705 | RIPK3 | Mus musculus | Ampicillin | PMID: | Backbone Marker:GE Dharmacon; Backbone Size:12300; Vector Backbone:pTRIPZ; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | D161N | 2022-12-09 12:06:45 | 0 | |
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pcDNA3-mCherry-TSC2 Resource Report Resource Website |
RRID:Addgene_192440 | mCherry-TSC2 | Homo sapiens | Ampicillin | PMID:35294906 | Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-12-09 12:04:16 | 0 | ||
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Ppst93-MCRaeo-NG mcrBDGA Resource Report Resource Website |
RRID:Addgene_192767 | Methyl coenzyme M reductase | Other | Ampicillin | PMID:36266535 | Backbone Size:4300; Vector Backbone:pMEV5; Vector Types:Synthetic Biology, Other, Heterologous expression in Methanococcus maripaludis; Bacterial Resistance:Ampicillin | 2022-12-09 12:04:17 | 0 |
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