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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pENTR-mCherry-attP(C31)-*BleoR
 
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RRID:Addgene_183752 FRT-mCherry-attP-BleoR-F3 Synthetic Kanamycin PMID:36313798 Backbone Size:2300; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin 2022-12-10 12:03:57 0
pBR_attB(C31)_FRT
 
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RRID:Addgene_183761 Ampicillin PMID:36313798 Backbone Size:5000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2022-12-10 12:03:57 0
pBR_attB(bxb)_lox
 
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RRID:Addgene_183762 Ampicillin PMID:36313798 Backbone Size:5000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin 2022-12-10 12:03:57 0
Bxb1_AJMA
 
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RRID:Addgene_183760 ASAP2f Synthetic Kanamycin PMID:36313798 Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2022-12-10 12:03:57 0
pBSSK p85 alpha (delta BH)
 
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RRID:Addgene_13431 p85 alpha (delta BH) Homo sapiens Ampicillin PMID:16880400 see author's map for more information. Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin BH domain replaced by flag tag. 2022-12-10 12:01:56 0
px330-Axin1
 
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RRID:Addgene_162543 sgRNA targeting Axin1 Mus musculus Ampicillin PMID:32814112 Backbone Marker:Feng Zhang lab (Addgene plasmid # 42230); Backbone Size:8500; Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin None 2022-12-10 12:02:59 0
pGEX p85a(N)-SH2
 
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RRID:Addgene_46464 p85a(N) Homo sapiens Ampicillin PMID:17588523 The molecular weight of the GST fusion protein is approximately 38.5 kDa Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin contains amino acids 321-434 2022-12-10 12:05:26 0
pGEX p85a(C)-SH2
 
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RRID:Addgene_46463 p85a(C) Homo sapiens Ampicillin PMID:17588523 The molecular weight of the GST fusion protein is approximately 38.4 kDa Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin contains amino acids 612-724 2022-12-10 12:05:26 0
iCAP-ROOT
 
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RRID:Addgene_192262 Synthetic construct for ROOT VP1 gene Synthetic Ampicillin PMID:36045288 Please note that the rep-cap construct is generated based on tTA-TRE amplification vector from Gradinaru lab (Addgene #103006; PubMed 28671695). Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2022-12-10 12:04:16 0
pGAL-flp(H305L)-NAT
 
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RRID:Addgene_160380 FLP1 Saccharomyces cerevisiae Ampicillin PMID: NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids. Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin H305L 2022-12-10 12:02:51 0
pGAL-SKI1-NAT
 
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RRID:Addgene_160382 SKI1 Saccharomyces cerevisiae Ampicillin PMID: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2022-12-10 12:02:51 0
UPB-pancreas (UPB7)
 
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RRID:Addgene_180787 hM4D(Gi) (partial ) Synthetic Ampicillin PMID:35296859 Backbone Marker:Edward Boyden; Backbone Size:6150; Vector Backbone:AAVrg-CAG-tdTomato-WPRE-SV40 (Addgene, Plasmid #59462); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2022-12-10 12:03:51 0
PRS_8-ChrimsonR-tdTomato
 
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RRID:Addgene_192587 ChrimsonR Synthetic Ampicillin PMID: Backbone Size:4095; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2022-12-10 12:04:17 0
PRS_8-eOPN3-mScarlet
 
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RRID:Addgene_192596 eOPN3 Synthetic Ampicillin PMID: Backbone Size:4098; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin 2022-12-10 12:04:17 0
pCDNA3-EMTB-TurboID-V5
 
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RRID:Addgene_190736 ensconsin Homo sapiens Ampicillin PMID:35952650 Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Microtubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882) 2022-12-10 12:04:13 0
hSyn-Aion-ts-mScarlet-ER
 
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RRID:Addgene_192577 Aion Synthetic Ampicillin PMID: Backbone Size:4372; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156C, T159G, G163A, V242R, T246N, N258Q, E273S 2022-12-10 12:04:17 0
pDB-His-MBP-mGSDMD
 
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RRID:Addgene_123365 GSDMD Mus musculus Kanamycin PMID:30361383 Backbone Marker:Berkeley Structural Genomics Center; Backbone Size:6494; Vector Backbone:pDB-His-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2022-12-09 12:01:21 0
pcDNA3-mCherry-Rheb(C181S)
 
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RRID:Addgene_192437 mCherry-Rheb(C181S) Homo sapiens Ampicillin PMID:35294906 Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Rheb Cys 181 mutated to Ser; abolishes C-terminal farnesylation. 2022-12-09 12:04:16 0
Flag-Gsdmd
 
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1+ mentions
RRID:Addgene_80950 Gsdmd Mus musculus Ampicillin PMID:27383986 Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2022-12-09 12:07:07 1
RCAS(A) mLef1 (CT#1141)
 
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RRID:Addgene_14021 Lef1 Mus musculus Ampicillin PMID: From Gabrielle Kardon. Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin 2022-12-09 12:02:14 0

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