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Species: Synthetic
Genetic Insert: FRT-mCherry-attP-BleoR-F3
Vector Backbone Description: Backbone Size:2300; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_183752 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183761 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression, Cre/Lox, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_183762 Copy
Species: Synthetic
Genetic Insert: ASAP2f
Vector Backbone Description: Vector Backbone:pBR322/pUC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_183760 Copy
Species: Homo sapiens
Genetic Insert: p85 alpha (delta BH)
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript SK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: see author's map for more information.
Proper citation: RRID:Addgene_13431 Copy
Species: Mus musculus
Genetic Insert: sgRNA targeting Axin1
Vector Backbone Description: Backbone Marker:Feng Zhang lab (Addgene plasmid # 42230); Backbone Size:8500; Vector Backbone:px330; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_162543 Copy
Species: Homo sapiens
Genetic Insert: p85a(N)
Vector Backbone Description: Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The molecular weight of the GST fusion protein is approximately 38.5 kDa
Proper citation: RRID:Addgene_46464 Copy
Species: Homo sapiens
Genetic Insert: p85a(C)
Vector Backbone Description: Backbone Marker:GE Healthcare Biosciences; Backbone Size:4900; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: The molecular weight of the GST fusion protein is approximately 38.4 kDa
Proper citation: RRID:Addgene_46463 Copy
Species: Synthetic
Genetic Insert: Synthetic construct for ROOT VP1 gene
Vector Backbone Description: Vector Backbone:pUC57-mini; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the rep-cap construct is generated based on tTA-TRE amplification vector from Gradinaru lab (Addgene #103006; PubMed 28671695).
Proper citation: RRID:Addgene_192262 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: FLP1
Vector Backbone Description: Backbone Marker:Yeast, Vol 14: 847–852 (1998); Backbone Size:6861; Vector Backbone:pBIS-GALkFLP(URA3); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: NAT cassette amplified from pLND46 using primers GGT GAT GAC GGT GAA AAC CTC and
CTG ATG CGG TAT TTT CTC CTT ACG . Vector flp(H305L)-URA3 (Yeast, Vol. 14: 847–852 (1998))amplified with oligos TGC GTA AGG AGA AAA TAC CGC & GTC AGA GGT TTT CAC CGT CAT outwards from the URA3 region to generate linear fragment with ends complementary to the NAT cassette. Both PCRs carried out using the Primestar MAX polymerase mix (Takara).The two products were then annealed using Gibson assembly, using 3X the amount of NAT PCR product compared to the flp PCR product. 2uL of the mix transformed into NEB 5-alpha competent cells, and transformants were miniprepped and checked for the correct marker replacement with BamH1-Nco1 restriction digest. New insert gives a smaller (1.2 kb) fragment than original vector (1.5kb fragment). Overexpression of flp(H305L) cures 2-micron plasmids.
Proper citation: RRID:Addgene_160380 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SKI1
Vector Backbone Description: Backbone Marker:Genetics. 2009 Sep; 183(1): 365–383.; Backbone Size:5643; Vector Backbone:pLND46; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160382 Copy
Species: Synthetic
Genetic Insert: hM4D(Gi) (partial )
Vector Backbone Description: Backbone Marker:Edward Boyden; Backbone Size:6150; Vector Backbone:AAVrg-CAG-tdTomato-WPRE-SV40 (Addgene, Plasmid #59462); Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_180787 Copy
Species: Synthetic
Genetic Insert: ChrimsonR
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192587 Copy
Species: Synthetic
Genetic Insert: eOPN3
Vector Backbone Description: Backbone Size:4098; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192596 Copy
Species: Homo sapiens
Genetic Insert: ensconsin
Vector Backbone Description: Backbone Size:5403; Vector Backbone:pCDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_190736 Copy
Species: Synthetic
Genetic Insert: Aion
Vector Backbone Description: Backbone Size:4372; Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192577 Copy
Species: Mus musculus
Genetic Insert: GSDMD
Vector Backbone Description: Backbone Marker:Berkeley Structural Genomics Center; Backbone Size:6494; Vector Backbone:pDB-His-MBP; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_123365 Copy
Species: Homo sapiens
Genetic Insert: mCherry-Rheb(C181S)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_192437 Copy
Species: Mus musculus
Genetic Insert: Gsdmd
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:pFlag-CMV-4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_80950 Copy
Species: Mus musculus
Genetic Insert: Lef1
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP(A); Vector Types:Retroviral, Other, avian expression; Bacterial Resistance:Ampicillin
References:
Comments: From Gabrielle Kardon.
Proper citation: RRID:Addgene_14021 Copy
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