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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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STAGR_gRNAScaffold_mU6 Resource Report Resource Website 1+ mentions |
RRID:Addgene_102844 | STAgR Insert gRNAScaffold_mU6 | Kanamycin | PMID:29702666 | Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:20 | 1 | |||
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STAGR_SAMScaffold_mU6 Resource Report Resource Website |
RRID:Addgene_102845 | STAgR Insert SAM_mU6 | Kanamycin | PMID:29702666 | Backbone Marker:Stricker Lab; Vector Backbone:PCR Template for STAgR Reactions; Vector Types:Other, PCR Template for STAgR Inserts; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:21 | 0 | |||
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STAGR_gRNAScaffold_h7SK Resource Report Resource Website |
RRID:Addgene_102842 | gRNAScaffold_h7SKpromoter | Kanamycin | PMID:29702666 | Vector Backbone:pcDNA1; Vector Types:Other, as PCR template; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:20 | 0 | |||
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VPR-ps-dSpCas9 Resource Report Resource Website |
RRID:Addgene_102855 | dSpCas9, pdDronpa1.2 | Synthetic | Ampicillin | PMID:28938067 | Backbone Marker:System Biosciences; Vector Backbone:pPB; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:21 | 0 | ||
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pCMV-5U-Venus-PSD-95-3U Resource Report Resource Website |
RRID:Addgene_102949 | PSD-95 | Mus musculus | Kanamycin | PMID:25948262 | Backbone Size:4141; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2022-04-22 03:15:22 | 0 | ||
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PresentER-SIINFEKL (mCherry) Resource Report Resource Website |
RRID:Addgene_102945 | SIINFEKL | Gallus gallus | Ampicillin | PMID:30499773 | Please visit https://www.biorxiv.org/content/early/2018/09/22/267047 for bioRxiv preprint. | Backbone Size:8200; Vector Backbone:PresentER; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin | OVA257-264 | 2022-04-22 03:15:22 | 0 |
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pHMR1A Resource Report Resource Website |
RRID:Addgene_102957 | MR1A cDNA | Homo sapiens | Ampicillin | PMID:24832684 | Backbone Marker:Invitrogen; Backbone Size:3908; Vector Backbone:pCR2.1-TOPO; Vector Types:Other, Cloning; Bacterial Resistance:Ampicillin | Stop codon has been removed by HindIII site | 2022-04-22 03:15:23 | 0 | |
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pMin1 Resource Report Resource Website |
RRID:Addgene_102959 | Tetracycline | PMID:8566811 | Artificial DNA for plasmid pMin1 GenBank: Z50148.1 | Backbone Size:2026; Vector Backbone:pRH144; Vector Types:Other, mini-transposon system suitable for sequencing, shuttling and gene fusions; Bacterial Resistance:Tetracycline | 2022-04-22 03:15:23 | 0 | |||
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pET22b_PfSSB_W166C Resource Report Resource Website |
RRID:Addgene_103001 | PfSSB_W166C | Other | Ampicillin | PMID:29466468 | Usage: The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, with two point mutations, C93A and W166C, for subsequent labeling at the 166 cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) or Cy3B per subunit. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77.W166C is the equivalent position to W88 in E. coli SSB, which was labeled with Cy3B for single molecule measurements of DNA unwinding using TIRF (Fili, N.; Mashanov, G.; Toseland, C. P.; Batters, C.; Wallace, M. I.; Yeeles, J. T. P.; Dillingham, M. S.; Webb, M. R.; Molloy, J. E., Visualizing DNA unwinding by helicases at the single molecule level. Nucleic Acids Res. 2010, 38, 4448-4457.) Protocol: See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. | Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C93A, W166C | 2022-04-22 03:15:24 | 0 |
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pET22b_PfSSB Resource Report Resource Website |
RRID:Addgene_103000 | PfSSB | Other | Ampicillin | PMID:29466468 | The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB, for subsequent labeling at the single, native cysteine with one IDCC (N-[2-(iodoacetamido)ethyl]-7-diethylaminocoumarin-3-carboxamide) per subunit. This adduct is then used as a fluorescent single-stranded DNA (ssDNA) biosensor, having ~20-fold fluorescence increase on binding ssDNA with very high affinity. PfSSB was expressed without the N-terminal apicoplast localisation sequence (76 amino acids) but amino acids are numbered for the full-length protein, that is the first amino acid in the expressed protein is M77. Protocol See Hedgethorne and Webb (2012), which is also followed for PfSSB purification, except with the omission of the PEI precipitation step. Hedgethorne, K.; Webb, M. R., Fluorescent SSB as a reagentless biosensor for single-stranded DNA. Methods Mol. Biol. 2012, 922, 219-233. | Vector Backbone:pET22B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | |
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pJH124 Resource Report Resource Website |
RRID:Addgene_102951 | APEX2-NES | Saccharomyces cerevisiae | Ampicillin | PMID:27274088 | Backbone Marker:ATCC; Backbone Size:7500; Vector Backbone:pRS425; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:23 | 0 | ||
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pZL1-ZF - myelin basic protein a (mbpa) Resource Report Resource Website |
RRID:Addgene_103007 | myelin basic protein | Danio rerio | Ampicillin | PMID:12112375 | Backbone Size:4308; Vector Backbone:pZL1; Vector Types:; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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GESTALT_pX330-v1 Resource Report Resource Website |
RRID:Addgene_103061 | integration of Cas9 with guide targeting GESTALT barcodes V1 to V5 | Homo sapiens | Ampicillin | PMID:27229144 | Derived from pX330-U6-Chimeric_BB-CBh-hSpCas9 | Vector Backbone:lentiCRISPR v2 (#52961); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:25 | 0 | |
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pCAGGS_3E5E_luciferase Resource Report Resource Website |
RRID:Addgene_103055 | Ebolavirus minigenome | Other | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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pSC101_TIMER Resource Report Resource Website |
RRID:Addgene_103057 | TIMER-bac | Synthetic | Kanamycin | PMID:25126781 | TIMER-bac was generated by exchanging TCC coding for serine 197 to ACC coding for threonine in DsRed.T3_S4T (Sörensen et al., 2003). PybaJ-Timer-bac was cloned into pUA66 with a pSC101 replicon, conferring kanamycin resistance. | Backbone Size:9262; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | mutations: S4T and S179T | 2022-04-22 03:15:25 | 0 |
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pCAGGS_L_EBOV Resource Report Resource Website |
RRID:Addgene_103052 | L gene of Ebola virus | Other | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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pCAGGS_VP30_EBOV Resource Report Resource Website |
RRID:Addgene_103051 | VP30 gene of Ebola virus | Other | Ampicillin | PMID:28807685 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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pCAGGS_HA-VP35_EBOV Resource Report Resource Website |
RRID:Addgene_103053 | VP35 | Other | Ampicillin | PMID:27252530 | Vector Backbone:pCAGGs; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:24 | 0 | ||
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pKSJ331 Resource Report Resource Website |
RRID:Addgene_103063 | Colicin E1 and E1 immunity protein | Other | Ampicillin | PMID:19029376 | Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene. | Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:25 | 0 | |
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pKSJ376 Resource Report Resource Website |
RRID:Addgene_103065 | colicin E1 immunity protein | Other | Ampicillin | PMID:27795317 | The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter. | Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2022-04-22 03:15:25 | 0 |
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