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Species: Arabidopsis thaliana
Genetic Insert: TIR-1
Vector Backbone Description: Vector Backbone:pDD356; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
References:
Comments: Pges-1::TIR::F2A::mTagBFP2 LGI
Proper citation: RRID:Addgene_172897 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIBN-rTetR
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_183919 Copy
Species: Arabidopsis thaliana
Genetic Insert: tdPCP-CIBN
Vector Backbone Description: Backbone Marker:Addgene #54642; Backbone Size:4592; Vector Backbone:tdTomato-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: under control of the weakened deltaTATA-CMV promoter for low level expression
Proper citation: RRID:Addgene_183937 Copy
Species: Arabidopsis thaliana
Genetic Insert: DHN_AtCor47
Vector Backbone Description: Vector Backbone:YK 02; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). See supplemental table 2 in our paper to see its performance in our assay. The negative control for this assay is pSecTag2 B which can be obtained from Thermo here https://www.thermofisher.com/order/catalog/product/V90020
Proper citation: RRID:Addgene_185254 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U3 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
References:
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173156 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2(olig)
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other, Other; Bacterial Resistance:Kanamycin
References:
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173733 Copy
Species: Arabidopsis thaliana
Genetic Insert: PYR1
Vector Backbone Description: Backbone Size:1774; Vector Backbone:pYTK084; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_183176 Copy
Species: Arabidopsis thaliana
Genetic Insert: Hen1
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:12028; Vector Backbone:pUASp-FMW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_104957 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1
Vector Backbone Description: Backbone Size:4091; Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_140573 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cyclin D3;1
Vector Backbone Description: Backbone Marker:15572; Vector Backbone:pGWB2; Vector Types:Plant Expression; Bacterial Resistance:Hygromycin
References:
Comments:
Proper citation: RRID:Addgene_140750 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cryptochrome 2
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160439 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cryptochrome 2
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160440 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cryptochrome 2
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160441 Copy
Species: Arabidopsis thaliana
Genetic Insert: VERNALIZATION1
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:3433; Vector Backbone:pQE30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_159532 Copy
Species: Arabidopsis thaliana
Genetic Insert: PIN2
Vector Backbone Description: Backbone Marker:SYSTEM BIOSCIENCES; Vector Backbone:PiggyBac; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_160045 Copy
Species: Arabidopsis thaliana
Genetic Insert: UBQ11 promoter
Vector Backbone Description: Backbone Size:2053; Vector Backbone:mUAV; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_153365 Copy
Species: Arabidopsis thaliana
Genetic Insert: LEC2 terminator
Vector Backbone Description: Backbone Size:2053; Vector Backbone:mUAV; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_153376 Copy
Species: Arabidopsis thaliana
Genetic Insert: NOS promoter
Vector Backbone Description: Backbone Size:2053; Vector Backbone:mUAV; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_153375 Copy
Species: Arabidopsis thaliana
Genetic Insert: ACT2 promoter
Vector Backbone Description: Backbone Size:2053; Vector Backbone:mUAV; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_153374 Copy
Species: Arabidopsis thaliana
Genetic Insert: HSP terminator
Vector Backbone Description: Backbone Size:2053; Vector Backbone:mUAV; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:
Proper citation: RRID:Addgene_153378 Copy
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