Plasmids   Select Another Resource Report Type

http://www.addgene.org/36425

Species:
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Other, Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette. A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_36425 Copy   

•   Source: Addgene

http://www.addgene.org/33298

Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3744; Vector Backbone:pBPXcm-1; Vector Types:Other, Plant expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_33298 Copy   

•   Source: Addgene

http://www.addgene.org/34914

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms. This vector does not contain a visual marker on the backbone and can be used when the gene of interest has visual phenotype. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34914 Copy   

•   Source: Addgene

http://www.addgene.org/34918

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: New version of Puromycin resistance vector for drug selection in worms. This vector replaces pBCN22-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34918 Copy   

•   Source: Addgene

http://www.addgene.org/34917

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: New version of Puromycin resistance vector for drug selection in worms. This vector replaces pBCN21-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34917 Copy   

•   Source: Addgene

http://www.addgene.org/34915

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Dual resistance (PuroR-NeoR) vector for drug selection following biolistic bombardment in worms. This vector contains a Pmyo-2::mCherry::myo-2_3'UTR pharyngeal marker on the backbone. Worm strains generated with vector can be crossed with strains generated with other visual markers such as in pBCN41-R4R3 which has a green fluorescent pharyngeal marker. This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34915 Copy   

•   Source: Addgene

http://www.addgene.org/34865

Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Other, Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.

Proper citation: RRID:Addgene_34865 Copy   

•   Source: Addgene

http://www.addgene.org/34863

Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Other, Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.

Proper citation: RRID:Addgene_34863 Copy   

•   Source: Addgene

http://www.addgene.org/34920

Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: New version of Neomycin resistance vector for drug selection in worms. This vector replaces pBCN24-R4R3 (main advantage of the new vector is compatibility with ccdB Survival cells, and the visual marker that expresses well in non-elegans Caenorhabditis species). This is a Gateway 3-fragment compatible destination vector. It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).

Proper citation: RRID:Addgene_34920 Copy   

•   Source: Addgene

http://www.addgene.org/35203

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11976; Vector Backbone:pBID-UASC-GRM; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Cterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.

Proper citation: RRID:Addgene_35203 Copy   

•   Source: Addgene

http://www.addgene.org/35202

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11145; Vector Backbone:pBID-UASC-G; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31transgenic Drosophila for GAL4 driven expression. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter(DSCP). Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35202 Copy   

•   Source: Addgene

http://www.addgene.org/35201

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11240; Vector Backbone:pBID-UASC-FG; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of Flag epitope fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). 3 copies of the Flag epitope is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35201 Copy   

•   Source: Addgene

http://www.addgene.org/35206

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11858; Vector Backbone:pBID-UASC-VG; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of mVenus fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). mVenus is fused to the N-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35206 Copy   

•   Source: Addgene

http://www.addgene.org/35204

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11858; Vector Backbone:pBID-UASC-GV; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of mVenus fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). mVenus is fused to the C-terminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35204 Copy   

•   Source: Addgene

http://www.addgene.org/35195

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:10033; Vector Backbone:pBID-G; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35195 Copy   

•   Source: Addgene

http://www.addgene.org/35199

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:13081; Vector Backbone:pBID-UAS-GGi; Vector Types:Other, Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven RNA inhibition (RNAi). 2 inverted gateway cloning cassettes are flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, hsp70 basal promoter. ftz intron facilitates RNA hairpin formation and expression. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinasemediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification. Please note that the inverted sequences present can cause rearrangements of this vector. Recommend miniprep only and confirm plasmid map subsequent to amplification or cloning. After gateway recombination, recommend amplification in Stbl2 cells [Invitrogen Cat. 10268-019] and combining minipreps for Drosophila injection. Addgene's sequencing results indicated a single nucleotide mismatch at bp# 5286 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect plasmid function. Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102

Proper citation: RRID:Addgene_35199 Copy   

•   Source: Addgene

http://www.addgene.org/16667

Species:
Genetic Insert: mTn7::Gateway cassette
Vector Backbone Description: Backbone Size:14263; Vector Backbone:pGRG37; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Grow at 32'C. Contains Gateway cassette with ccdB gene. See author's protocol for more information. This plasmid is designed to insert DNA into the chromosome of Enterobacteria in a manner that doesn't require a drug resistance marker. This plasmid uses the site-specific recombination machinery of the bacterial transposon Tn7. The backbone of the plasmid is the easily curable temperature sensitive mutant of pSC101.

Proper citation: RRID:Addgene_16667 Copy   

•   Source: Addgene

http://www.addgene.org/169184

Species: Synthetic
Genetic Insert: nsp12-His6-3xFlag/nsp7-Linker-nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).

Proper citation: RRID:Addgene_169184 Copy   

•   Source: Addgene

http://www.addgene.org/169185

Species: Synthetic
Genetic Insert: nsp12-His6-3xFlag/nsp7/nsp8
Vector Backbone Description: Vector Backbone:pBIG2abc (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).

Proper citation: RRID:Addgene_169185 Copy   

•   Source: Addgene

http://www.addgene.org/17386

Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8832; Vector Backbone:pQCXIP; Vector Types:Mammalian Expression, Retroviral, Other, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_17386 Copy   

•   Source: Addgene