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Species: Mus musculus
Genetic Insert: Activating transcription factor 4
Vector Backbone Description: Backbone Marker:Systems Biosciences Inc; Backbone Size:6592; Vector Backbone:pCDF1-MCS2-EF1-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101795 Copy
Species: Mus musculus
Genetic Insert: Fibroblast growth factor 21 promoter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101797 Copy
Species:
Genetic Insert: S. pyogenes Cas9 codon optimized for expression in S. cerevisiae.
Vector Backbone Description: Vector Backbone:pRS414; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101725 Copy
Species: Other
Genetic Insert: CatRhAC
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101722 Copy
Species: Other
Genetic Insert: CatRhGC
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4568; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101720 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101817 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101818 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101815 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101816 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101811 Copy
Species: Homo sapiens
Genetic Insert: BAF (BANF1)
Vector Backbone Description: Backbone Size:5517; Vector Backbone:custom bacterial expression vector; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101779 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2α1; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101708 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_101709 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_101705 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:GoldenBraid 2.0; Vector Backbone:pDGB2Ω1; Vector Types:Yeast Expression; Bacterial Resistance:Spectinomycin
References:
Comments:
Proper citation: RRID:Addgene_101706 Copy
Species: Homo sapiens
Genetic Insert: CETN2 Homology Arms with mTagRFPT-linker
Vector Backbone Description: Vector Backbone:pUC57; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human CETN2 in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: SGLRS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101781 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101821 Copy
Species: Homo sapiens
Genetic Insert: TUBA1B Homology Arms with mTagRFPT-linker
Vector Backbone Description: Backbone Marker:New England BioLabs; Vector Backbone:pUC19; Vector Types:Mammalian Expression, CRISPR, Other, Donor Template; Bacterial Resistance:Ampicillin
References:
Comments: This plasmid has been used with locus-specific CRISPR/Cas9 to add a mTagRFP-T tag to the N-terminus of human TUBA1B in WTC human induced pluripotent stem cells by the Allen Institute for Cell Science. Linker (AA) sequence: GGSGGS. After protein tagging using this donor template plasmid and CRISPR/Cas9 reagents, transfected cells may exhibit varying intensity levels of fluorescence, likely due to editing precision. To obtain cells of uniform intensity levels, see our protocol for fluorescence-assisted cell sorting and subcloning of transfected cells (https://www.allencell.org/instructional-videos-and-tutorials-for-cell-methods.html). Further, we recommend PCR-based assays for identifying precisely edited clones as previously described (https://www.molbiolcell.org/doi/abs/10.1091/mbc.e17-03-0209). For more information on the entire plasmid collection, please see https://www.addgene.org/allen-institute-cell-science/ .
Proper citation: RRID:Addgene_101785 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101750 Copy
Species: Synthetic
Genetic Insert: crRNAs targeting GFP and mCherry
Vector Backbone Description: Backbone Size:2870; Vector Backbone:pCRRNAcos; Vector Types:CRISPR, Synthetic Biology; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_101804 Copy
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