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Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Other, Gateway Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK.
Proper citation: RRID:Addgene_18668 Copy
Species:
Genetic Insert: Gateway cassette A
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_18656 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.
Proper citation: RRID:Addgene_18700 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag.
Proper citation: RRID:Addgene_18701 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, Other, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18704 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, Other, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18705 Copy
Species: Chloramphenicol resistance gene (CmR) - ccdB gene
Genetic Insert: Chloramphenicol resistance gene (CmR) - ccdB gene
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9400; Vector Backbone:pLenti6/UbC/V5-Dest; Vector Types:Mammalian Expression, Lentiviral, Other, Gateway; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: V5 tag deleted
Proper citation: RRID:Addgene_24179 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Soriano Lab (Addgene Plasmid# 21714); Backbone Size:9846; Vector Backbone:pRosa26-1; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette.
Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418
Proper citation: RRID:Addgene_24418 Copy
Species: reporter gene Venus
Genetic Insert:
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10474; Vector Backbone:pGreen H-Pelican; Vector Types:Other, reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24568 Copy
Species: phage phiC31
Genetic Insert: phiC31 attB site
Vector Backbone Description: Backbone Size:4670; Vector Backbone:pDEST; Vector Types:Other, Drosophila transgenesis; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Gateway destination vector with phiC31 attB site, for Drosophila transformation into attP landing sites.
Proper citation: RRID:Addgene_30325 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Khavari Lab; Backbone Size:11100; Vector Backbone:LZRS-RfA; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_31601 Copy
Species:
Genetic Insert: TALE Nuclease, NN for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32191 Copy
Species:
Genetic Insert: TALE Nuclease, HD for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32192 Copy
Species:
Genetic Insert: TALE Transcriptional Activator Backbone, HD for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32188 Copy
Species:
Genetic Insert: TALE Nuclease, NI for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32189 Copy
Species:
Genetic Insert: TALE Transcriptional Activator Backbone, NG for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32186 Copy
Species:
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pBSC5; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Please see http://www.everyvector.com/sequences/show_public/12784 for additional plasmid map.
Proper citation: RRID:Addgene_32317 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Mo Bi Tec; Vector Backbone:PET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_32485 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:NA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_37843 Copy
Species:
Genetic Insert: Gateway Cassette
Vector Backbone Description: Vector Backbone:pMartini; Vector Types:Other, Gateway Conversion Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: This vector can be used for conversion of any cloning vector into a gateway destination vector and facilitate the generation of in-frame fusions with a gateway cassette.
A,B or C are reading frames; R1-R2 or R2-R1 indicate the gateway cassette orientation.
Please see the associated publication for more information: Wang J-W, Beck ES, McCabe BD (2012) A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila. PLoS ONE 7(7): e42102 http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0042102
Proper citation: RRID:Addgene_36436 Copy
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