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Plasmid Name
mCHOP10.pCDNA1
RRID:Addgene_21899 RRID Copied  
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RRID:Addgene_21899
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Plasmid Information

URL: http://www.addgene.org/21899

Proper Citation: RRID:Addgene_21899

Insert Name: CHOP10

Organism: Mus musculus

Bacterial Resistance: Ampicillin and Tetracycline

Defining Citation: PMID:1547942

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pcDNA1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Tetracycline

Comments: Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence. Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.

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Data and Source Information

Source: Addgene