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URL: http://www.addgene.org/42601
Proper Citation: RRID:Addgene_42601
Insert Name: daf-15
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin and Tetracycline
Defining Citation: PMID:22278922
Vector Backbone Description: Backbone Size:2790; Vector Backbone:L4440; Vector Types:RNAi; Bacterial Resistance:Ampicillin and Tetracycline
Comments: This plasmid can be kept in DH5alpha cells but does not work for RNAi in that strain. For RNAi, please transform DNA into the HT115 strain. Note: Addgene's quality control sequencing has found that the deposited clone contains a T to C mismatch with respect to the C. elegans N2 reference daf-15 sequence, which would cause a V797A change in the coded protein. According to the depositing lab, this point mutation does not affect RNAi feeding; however, its possible effects in other contexts are not known.
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Source: Addgene
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