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URL: http://www.addgene.org/26415
Proper Citation: RRID:Addgene_26415
Insert Name: attR4-ccdB-attR2
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:15489340
Vector Backbone Description: Backbone Size:8623; Vector Backbone:pDP#MM016b; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow. The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.
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Source: Addgene
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