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URL: http://www.addgene.org/129202
Proper Citation: RRID:Addgene_129202
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Vector Backbone Description: Backbone Marker:Dirk Landgraf; Backbone Size:5056; Vector Backbone:pDML112; Vector Types:Yeast Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: This is the empty basic yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN147. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin.
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Source: Addgene
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