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URL: http://www.addgene.org/114850
Proper Citation: RRID:Addgene_114850
Insert Name: AT1G75640
Organism: Arabidopsis thaliana
Bacterial Resistance: Ampicillin
Defining Citation: PMID:29320478
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47051); Vector Backbone:pECIA14; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Pentameric rat COMP helix, Alkaline Phosphatase (human, placental), Flag, and hexahistidine tags. Insert Protein Sequence: SSETQALTSFKLSLHDPLGALESWNQSSPSAPCDWHGVSCFSGRVRELRLPRLHLTGHLSPRLGELTQLRKLSLHTNDINGAVPSSLSRCVFLRALYLHYNSFSGDFPPEILNLRNLQVLNAAHNSLTGNLSDVTVSKSLRYVDLSSNAISGKIPANFSADSSLQLINLSFNHFSGEIPATLGQLQDLEYLWLDSNQLQGTIPSALANCSSLIHFSVTGNHLTGLIPVTLGTIRSLQVISLSENSFTGTVPVSLLCGYSGYNSSMRIIQLGVNNFTGIAKPSNAACVNPNLEILDIHENRINGDFPAWLTDLTSLVVLDISGNGFSGGVTAKVGNLMALQELRVANNSLVGEIPTSIRNCKSLRVVDFEGNKFSGQIPGFLSQLRSLTTISLGRNGFSGRIPSDLLSLYGLETLNLNENHLTGAIPSEITKLANLTILNLSFNRFSGEVPSNVGDLKSLSVLNISGCGLTGRIPVSISGLMKLQVLDISKQRISGQLPVELFGLPDLQVVALGNNLLGGVVPEGFSSLVSLKYLNLSSNLFSGHIPKNYGFLKSLQVLSLSHNRISGTIPPEIGNCSSLEVLELGSNSLKGHIPVYVSKLSLLKKLDLSHNSLTGSIPDQISKDSSLESLLLNSNSLSGRIPESLSRLTNLTALDLSSNRLNSTIPSSLSRLRFLNYFNLSRNSLEGEIPEALAARFTNPTVFVKNPGLCGKPLGIECPNVRRRRRRKL
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Source: Addgene
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