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URL: http://www.addgene.org/113758
Proper Citation: RRID:Addgene_113758
Insert Name: gateway cassette
Organism: Other
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:31523744
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3048; Vector Backbone:pGEM-T easy; Vector Types:Other, Gateway Destination Vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Use this vector for 3-way multi-site Gateway reactions with two regions of homology flanking an insert - this final vector can be used as a template for repair (homology-directed repair) after CRISPR double-strand break. This plasmid was constructed by A-tailing the Gateway cassette (R1R2) and blunt/TA Ligase reaction with pGEM-T Easy to create pGEM-gate.
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Source: Addgene
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